Abstract:Circular RNAs (circRNAs) are a class of competing endogenous RNAs (ceRNAs) with a closed loop structure, which regulate gene expression by competitively adsorbing microRNAs (miRNAs). circRNA dysregu-lation is closely related to the proliferation, differentiation and invasion of cancer cells. This study uses ceRNAs as an entry point to reveal the potential pathogenic mechanism of circRNAs in gastric cancer cells, and provide potential clinical diagnosis and treatment targets for gastric cancer and also new ideas for the scientific research of gastric cancer based on the joint application of bioinformatics analysis and multiple data-bases. First, by mining the differentially expressed circRNAs, miRNAs and mRNAs of gastric cancer cells, the circRNA-miRNA-mRNA ceRNA network was constructed. Then, the network topological attribute analy-sis was used to search the core nodes, and the core node-mediated subnetwork was extracted from the original ceRNA network. Finally, functional enrichment analysis and survival analysis were used to analyze the biological function of the network and mine prognostic genes, respectively. The results showed that a total of 6 core nodes (hsa_circ_0008468, hsa_circ_0005822, hsa_circ_0025842, hsa-miR-940, hsa-miR-944, and hsa-miR-515-5p) were mined out. A subnetwork containing 8 pairs of circRNA-miRNA and 539 pairs of miRNA-mRNA was extracted from the original ceRNA network. Results of functional enrichment indicated that the subnetwork was involved in multiple cancer-related biological processes including metabolic pathways, cAMP signaling pathways, and pathways in cancer, etc. Survival analysis revealed that 14 genes such as ACO2, E2F8, GHR, ITIH5 in the subnetwork were significantly related to the prognosis, indicating that the three core circRNAs-mediated ceRNA network is closely related to the occurrence, development and prognosis of gastric cancer.
引用本文:
李 让, 廖苑君, 孙胜南, 蓝树金, 赵小蕾, 覃继恒, 饶绍奇. 联合TCGA和GEO数据库构建和分析胃癌中circRNA相关的ceRNA网络[J]. 生命科学研究, 2020, 24(4): 275-283.
LI Rang, LIAO Yuan-jun, SUN Sheng-nan, LAN Shu-jin, ZHAO Xiao-lei, QIN Ji-heng, RAO Shao-qi. Construction and Analysis of circRNA-related ceRNA Network in Gastric Cancer by Combining TCGA and GEO Databases. Life Science Research, 2020, 24(4): 275-283.