Abstract:Scanning electron microscope (SEM) is an important way to study the development and morphoge-nesis of cotton stomata. Herein, advantages and disadvantages in cotton pore observation with SEM, environ-mental scanning electron microscope (ESEM), cryo-SEM, and print-SEM were analyzed by comparing horizon-tally their sampling methods, including the sample collection, preparation time and microscopy results. SEM proved to have the longest sample preparation time, some water loss in samples, but the best image contrast. ESEM took the shortest time to make samples, but had serious water loss in samples and poor image contrast. The morphology of the samples was consistent with that observed by the SEM biological sampling method. Cryo-SEM took a long time to prepare samples, and there was no water loss in the samples, but poor image contrast. The sample preparation time for print-SEM was short, with no water loss and a better image contrast and the sample presented the morphology of the reverse cryo-SEM, but the charge was more due to the tip discharge. This study revealed what kind of stomatal image data could be obtained by different SEM sampling methods, and may provide some references for research on drought-resistant basis and application of cotton.
引用本文:
刘 恺, 密玲煜, 刘玲玲, 李 洋. 不同扫描电镜制样方法观察棉花叶片气孔[J]. 生命科学研究, 2020, 24(4): 293-300.
LIU Kai, MI Ling-yu, LIU Ling-ling, LI Yang. Different SEM Sample Preparation Methods to Observe the Stomata of Cotton Leaves. Life Science Research, 2020, 24(4): 293-300.