Abstract:A molecular epidemiological survey was conducted among Han and Mongolian women during pregnancy in Chifeng City to study the genetic polymorphism distribution of key folate metabolism enzymes 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T, A1298C, and methionine synthase reductase (MTRR) A66G. A total of 853 pregnant women were analyzed in this study, including 647 Han people and 206 Mongolians. Exfoliated oral mucosal epithelial cells were collected, genomic DNA was extracted, and the polymorphisms of MTHFR C677T, A1298C and MTRR A66G were detected by real-time quantitative PCR. The results showed that the genetic polymorphism distribution of the enrolled subjects conformed to the genetic balance. The genotype frequencies of MTHFR C677T CC, CT and TT in Han women during pregnancy were 15.61%, 52.40% and 31.99%, respectively, and those in Mongolian women during pregnancy were 23.79%, 50.97% and 25.24%, respectively, and the difference between them was statistically significant (P<0.05). The genotype frequencies of MTHFR A1298C AA, AC and CC in Han women during pregnancy were 76.04%, 22.10% and 1.86%, respectively, and those in Mongolian women during pregnancy were 74.27%, 23.30% and 2.43%, respectively, and the difference was not statistically significant (P>0.05). The genotype frequencies of MTRR A66G AA, AG and GG in Han women during pregnancy were 58.42%, 36.63% and 4.95%, respectively, and those in Mongolian women during pregnancy were 50.48%, 40.78% and 8.74%, respectively, and the difference was statistically significant (P<0.05). The two loci, MTHFR C677T and A1298C had six combinations, with CT/AA the highest frequency, followed by TT/AA, CT/AC, CC/AA, CC/AC, CC/CC and there were no CT/CC, TT/AC and TT/CC combinations. They were in complete linkage disequilibrium. By this study, the population genetic characteristics of MTHFR and MTRR genetic polymorphisms were obtained in pregnant Han and Mongolian women in Chifeng City, providing a basis for guiding the scientific supplementation of folic acid nutrition and implementing personalized pregnancy care.
引用本文:
苏亚力, 李耀辉, 乌兰托娅, 江玉华, 崔贵虎, 王 改, 华永娟, 胡季芳, 鲁衍强. 赤峰市汉族和蒙古族孕期女性MTHFR与MTRR的基因多态性分布研究[J]. 生命科学研究, 2020, 24(4): 271-274.
SU Ya-li, LI Yao-hui, ULAN To-ya, JIANG Yu-hua, CUI Gui-hu, WANG Gai, HUA Yong-juan, HU Ji-fang, LU Yan-qiang. Study of Genetic Polymorphisms of MTHFR and MTRR in Han and Mongolian Women During Pregnancy in Chifeng City. Life Science Research, 2020, 24(4): 271-274.