Abstract：Abstract: Reactive oxygen species (ROS) play an important role in the occurrence and development of diseases, such as non-alcoholic fatty liver, cardiovascular disease, cancer, diabetes. HepG2 cells are commonly used cell models to evaluate the protective effects of antioxidants on oxidative damage of living cells. In order to investigate the effect and mechanism of fisetin on scavenging ROS induced by H2O2, HepG2 cells were randomly divided into the control group, solvent control group, H2O2 model group, fisetin intervention group (fisetin+H2O2) and fisetin treatment group (fisetin). Then the cell viability and intracellular ROS levels in different intervention groups were measured. The expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein (Keap1) and phase II enzymes such as heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), NAD-(P)H quinone oxidoreductase 1 (NQO1) were detected. The role of Nrf2 in the process of scavenging ROS by fisetin was further clarified by constructing Nrf2 knockdown cell lines. It was found that compared with the H2O2 model group, the cell survival rate of fisetin intervention group increased significantly. Fisetin could reduce ROS, up-regulate Nrf2 and HO-1 protein expression levels and down-regulate Keap1 protein expression in HepG2 cells induced by H2O2. After Nrf2 was stably knocked down, the ROS level increased. The results showed that fisetin could induce HO-1 expression by activating Keap1/Nrf2/antioxidant response element (ARE) pathway, thus playing a protective role in the process of antioxidantion.
引用本文:
张妍薇, 郑 皖, 杨人贵, 陈静芳, 向晓婧, 陈继华. Fisetin对H2O2诱导细胞内ROS的清除作用及机制探讨[J]. 生命科学研究, 2019, 23(6): 437-443.
ZHANG Yan-wei, ZHENG Wan, YANG Ren-gui, CHEN Jing-fang, XIANG Xiao-jing, CHEN Ji-hua. Scavenging Effects of ROS by Fisetin in H2O2-induced Cells and Its Mechanism. Life Science Research, 2019, 23(6): 437-443.
2019, Vol. 23 
