Abstract:Abstract: Mesenchymal stem/stromal cells (MSCs), which reside mainly in bone marrow, are known to be recruited to tumor sites and constitute the tumor microenvironment. Metabolism plays an important role in cancer progression. In the cancer microenvironment, cells including mesenchymal and cancer cells have a shift between glycolysis and mitochondrial oxidative phosphorylation. However, how the interaction of cancer cells and bone marrow MSCs (BMSCs) affects the metabolism of cells and cancer progression remains to be elucidated. Herein, an animal model was established by subcutaneously co-injecting BMSCs and murine Lewis lung cancer (LLC) cells into C57BL/6 mice. BMSCs and LLC cells were isolated from primary tumor sites, and RNA sequencing was performed to obtain the transcriptomic profiles in BMSCs and LLC cells under the cancer microenvironment. The result revealed that the upregulated genes in BMSCs were enriched in the metabolic pathway. The Pathway-Act-Network was built according to differentially expressed molecules, and the results showed that the core predicted pathways in the network of BMSCs were metabolic pathways, MAPK signaling pathway and HIF-1 signaling pathway. However, the metabolic pathways were suppressed in LLC cells in cancer microenvironment. Inhibition of glycolysis could reduce the tumor growth in vivo. The study concluded that BMSCs increase glycolysis, promoting growth of cancer cells through reverse Warburg effect. Metabolic reprogramming of BMSCs in cancer microenvironment would determine the fate of cancer cells.
引用本文:
赛步青,徐嘉齐,王路娟,郑乐亮,唐敬群. 间充质干细胞通过分解代谢糖酵解促进肺癌细胞生长(英文)[J]. 生命科学研究, 2019, 23(6): 431-436.
SAI Bu-qing, XU Jia-qi, WANG Lu-juan, ZHENG Le-liang, TANG Jing-qun. Mesenchymal Stem Cells Promote Growth of Lung Cancer Cells Through Catabolic Glycolysis. Life Science Research, 2019, 23(6): 431-436.