Abstract:Abstract: To evaluate the clinical significance and diagnostic value of miRNA-195-5p in liver hepatocellu-lar carcinoma (LIHC), the expression and prognostic value of miRNA-195-5p and the network of its target genes in LIHC were analyzed using bioinformatics tools. The expression profiles in LIHC tissues were analyzed using The Cancer Genome Atlas (TCGA) database. The relationship between miRNA-195 and the survival of LIHC was analyzed using the Kaplan-Meier plotter online tools. miRWalk 2.0 with 12 online target gene prediction databases was used to attain the prospective target genes. Gene Ontology (GO) analysis, enrichment of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein-protein interaction (PPI) analysis were performed to provide an overview of the functions of miRNA-195-5p in LIHC. A set of publicly accessible databases were used for validation of hub genes. Based on miRNA-Seq data in the TCGA database, miRNA-195-5p was shown to be downregulated in LIHC tissues. Low miRNA-195 expression was associated with an unfavorable overall survival of LIHC. Protein phosphorylation, positive regulation of transcription from RNA polymerase Ⅱ promoter and negative regulation of transcription from RNA polymerase Ⅱ promoter were highlighted as the most enriched GO terms. KEGG analysis indicated that these genes were mainly involved in cancer-related pathways. Six genes, POLR2A, MIB1, MAPK3, ACACA, ASH1L and MAP3K3, were defined as hub genes in the PPI network. Among them, only MAPK3 showed an up-regulated pattern in both mRNA and protein levels. Furthermore, high MAPK3 expression was associated with an unfavorable overall survival of LIHC. The bioinformatics analysis suggests that down-regulated miRNA-195-5p may play an essential regulatory role in the biological process of LIHC, which may be associated with expression upregulation of its target gene MAPK3. The results provide clues for studying potential diagnostic biomarkers of LIHC.
引用本文:
宋秀道, 马 锦. 基于生物信息学分析miRNA-195-5p在肝癌中的表达及其临床意义[J]. 生命科学研究, 2019, 23(2): 100-107.
SONG Xiu-dao, MA Jin. Expression of miRNA-195-5p in Hepatocellular Carcinoma and Its Clinical Significance: Evidence from Bioinformatics Analysis. Life Science Research, 2019, 23(2): 100-107.