Abstract:Abstract: In vivo cells live in a complex microenvironment, which plays a vital role in maintaining cell functions. Many investigations have revealed that in vivo cells respond to various gradient mechanical stimuli, which can regulate cell behavior and guide cell fate. Hydrogels are widely applied to engineering cell microenvironment due to their hydrated environment and tunable properties (e.g., mechanical, chemical and biocompatible), similar to the native extracellular matrix. Herein, the methods of construction, detection and characterization of cell mechanical microenvironment, especially stiffness and tensile/compressive stress, were described. The latest research progress of hydrogels, which are selected as an appropriate matrix for mimicking 2D and 3D cell mechanical microenvironment, was introduced. What’s more, the new technologies and methods for the construction of gradient mechanical microenvironment and its implications in controlling the development, healing, and homeostasis attainment were highlighted. And, some challenges and the expectations in the application of gradient mechanical microenvironment in tissue remodeling and regeneration medicine were also proposed.
引用本文:
王 琳, 何 楠, 李昱辉, 周宝珍, 李 娜. 细胞梯度力学微环境体外构建的研究[J]. 生命科学研究, 2019, 23(2): 146-151.
WANG Lin, HE Nan, LI Yu-hui, ZHOU Bao-zhen, LI Na. Engineering of Cell Mechanical Gradient Microenvironment and Its Applications. Life Science Research, 2019, 23(2): 146-151.