Abstract:Abstract: To study the epigenetic changes in replicative senescence, the ATAC-seq was performed in young (PD26) and senescent (PD55) 2BS cells. After sequencing, the results were analyzed by Bowtie2, FastQC, MACS, deepTools, phastCons, R language, HOMER and Gene Ontology (GO). The enrichment sites of open chromatin regions were highly conserved. Although the open regions were mainly enriched between the promoter and transcription start site (TSS), the enrichments in the young and the old are not the same, varying with aging of 2BS cells. Furthermore, GO analysis was performed to analyze the differentially expressed genes in different ages of 2BS cells. The genes were mainly clustered in cellular process, metabolic process, biological regulation, binding and so on. These results showed that the aging process was accompanied by declined degree of chromatin openness and decreased level of transcriptional regulation, which revealed the close relationship between the replicative senescence and transcriptional regulation.
引用本文:
宋 乔, 王亚琦, 侯玉丽, 刘 静, 张晓敏, 曹 敏, 王培昌. 衰老细胞染色质开放变化的初步分析[J]. 生命科学研究, 2020, 24(2): 109-117.
SONG Qiao, WANG Ya-qi, HOU Yu-li, LIU Jing, ZHANG Xiao-min, CAO Min, WANG Pei-chang. Chromatin Accessibility Dynamics in Replicative Senescence. Life Science Research, 2020, 24(2): 109-117.