Abstract:Abstract: To explore the protective effect of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on neonatal porcine islet cell clusters (NICCs) exposed to hypoxia by paracrine potential, the NICCs in hUC-MSC-conditioned medium (hu-MSC-CM) and RPMI1640 medium (control group) were cultured under 20% O2 or 1% O2 conditions. The viability and function of NICCs in different media were studied by fluorescence activated cell sorter (FACS) and the extracellular flux assay, respectively. The expression of hypoxia survival related proteins was detected by Western-blot. Compared with the control groups, the results showed that the survival ratio, viability, and function were improved significantly in NICCs cultured in hu-MSC-CM (P<0.05). The expression of hypoxia-inducible factor 1α (HIF-1α) was up-regulated, and meanwhile, the expression of apoptosis and autophagy associated proteins decreased in Western-blot assay. Experimental results showed that MSCs can improve the viability and function via the paracrine effect.
引用本文:
伍君子, 马小倩, 杨策军, 容鹏飞, 聂 唯, 王 维. 人脐带间充质干细胞条件培养基对缺氧胰岛的保护作用[J]. 生命科学研究, 2018, 22(6): 461-467.
WU Jun-zi, MA Xiao-qian, YANG Ce-jun, RONG Peng-fei, NIE Wei, WANG Wei. Protective Effect of Conditioned Medium from Human Umbilical Cord-derived Mesenchymal Stem Cells on NICCs Exposed to Hypoxia. Life Science Research, 2018, 22(6): 461-467.