Abstract:Abstract: Delta sleep inducing peptide (DSIP) has a low relative molecular mass and its metabolic half-life is short in vivo, which greatly limits its clinical application. Here the protein transduction domain (PTD), hu-man serum albumin (HSA), linker and DSIP were used to express the recombinant protein named PHLD. The recombination plasmid pPIC9K/PHLD was obtained by PCR amplification with the plasmid pPIC9K/HSA as a template. After PCR identification, restriction endonuclease analysis and DNA sequencing, the recombinant plasmid was transferred into the His-deficient mutant strain GS115. The high-expression strain named No.lut1211 was selected by screening on MM/MD plates with G418, and high-purity fusion proteins were obtained. This would lay the foundation for further study and application of DSIP.
引用本文:
张新国, 郭广君, 李 坤, 孙巧云, 郭思佳, 马小弟, 马国艳. PHLD融合蛋白在毕赤酵母中的构建和表达[J]. 生命科学研究, 2018, 22(6): 475-482.
ZHANG Xin-guo, GUO Guang-jun, LI Kun, SUN Qiao-yun, GUO Si-jia, MA Xiao-di, MA Guo-yan. Construction and Expression of PHLD Fusion Protein in Pichia pastoris. Life Science Research, 2018, 22(6): 475-482.