Abstract:High blood cholesterol is a serious threat to human health. The cholesterol-reducing rates of 22 Enterococcus faecium strains were screened by the saponification-colorimetric method in vitro, and were found to range from 85.93%~21.25%. Three strains with better bile salt tolerant activity were selected for further studies. Among them, the acid resistance of E. faecium SP5-6L was poor while strains JS2 and ML13-5 were able to survive at pH 2.0 and pH 3.0 for 3 h and in 0.3%, 0.5% and 1% bile salt for 12 h without losing their viability. All three strains had a high activity of bile salt hydrolase. The cholesterol-reducing rate of strain ML13-5 was increased from 52.71% to 68.01% at 0.3% bile salt. In addition, three strains had no hemolysis, but with a different sensitivity to ten kinds of antibiotics. This preliminary study on the mechanism of cholesterol reduction by E. faecium provides basis for its further utilization and for development of related functional foods and drugs.
引用本文:
吴 边, 柳陈坚, 李雅迪, 尚 云, 李晓然. 屎肠球菌降胆固醇作用的初步研究[J]. 生命科学研究, 2018, 22(4): 305-310.
WU Bian, LIU Chen-jian, LI Ya-di, SHANG Yun, LI Xiao-ran. Preliminary Study on Cholesterol Reduction by Enterococcus faecium. Life Science Research, 2018, 22(4): 305-310.