Abstract��Abstract: Heat shock transcription factor 4 (HSF4) is a member of HSF family. Recently, it was reported that HSF4 not only regulates the expression of heat shock protein(HSP), but also directly or indirectly controls many non-heat shock gene expressions, involved in cell growth and some physiological and pathological processes. The aim of this study was to clarify the target genes regulated by HSF4 in the hepatoma cell line SMMC-7721. The potential target genes of HSF4 were detected by chromatin immunoprecipitation sequencing (ChIP-Seq) method. Then they were analyzed by gene ontology (GO) to explore the biological function of HSF4. In ChIP-Seq analysis, 1 726 binding peaks were identified, and among them, 102 were located in the promoter region. The results of GO analysis indicated that these target genes were associated with cell development, proliferation and response to stimuli in biological processes. Some of these genes could bind proteins or nucleic acids and activate proteins. They were also involved in drug metabolism, xenobiotics metabolism and chemical carcinogenesis pathways. Furthermore, MEME4.12.0 software was used to identify the HSF4 binding DNA motifs, and three motifs were discovered for coding genes and the other three motifs for lncRNAs. These results clarify the target genes of HSF4, and provide the experimental basis for further exploring the roles of HSF4 in tumorigenesis of liver cancer.
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���꺣, ���콾, ���ҳ�, �����, �� Ⱥ. �ΰ�SMMC-7721ϸ��ϵHSF4���صİл���ͼ����[J]. ������ѧ�о�, 2018, 22(3): 201-207.
MA Ru-hai, WANG Tian-jiao, PAN Zhong-cheng, ZHAO Yu-jie, HE Qun. Target Gene Mapping Analysis of HSF4 Regulation in Hepatocellular Carcinoma SMMC-7721 Cells. Life Science Research, 2018, 22(3): 201-207.