Abstract:Abstract: Bioinformatics analysis methods were used to screen the key genes involved in differentiation of cardiomyocytes from human induced pluripotent stem cells (iPSCs). The expression data of high-throughput sequencing of human iPSCs differentiation into cardiomyocytes were collected from the Gene Expression Om-nibus (GEO) database, including sample data of 4 differentiation stages, namely undifferentiated iPSCs (D0), mesoderm cells (D2), early cardiomyocytes (D7), and cardiomyocytes (D14). The differentially expressed genes of D0, D2, D7, and D14 were analyzed by the R package, and their functions and signal pathways were ana-lyzed. The protein-protein interaction (PPI) network of the differentially expressed genes was constructed. The coexpressed genes were clustered and divided into different gene modules by the R package weighted gene coexpression network analysis (WGCNA). The Cytoscape software was used for visualization and enrichment analysis of the interaction network in important gene module. A total of 917 differentially expressed genes were obtained in the D0, D2, D7 and D14 samples. The top 10 genes screened based on PPI network were FPR2, ADCY2, CXCR2, CXCR4, PF4, GALR1, GAL, CXCL5, CXCL6, and HCAR3, among which FPR2, CXCR2, CXCR4, and PF4 were all enriched in G-protein-coupled receptor-ligand binding. According to the interaction between genes in modules, BMP5 was found to be at the core of regulating myocardial develop-ment. Through bioinformatics analysis of the expression profile GSE137920, the key genes in differen-tiation of iPSCs into cardiomyocytes were screened out. The results may be helpful for in-depth research on the molecular mechanism of cardiac differentiation and development.
引用本文:
涂思梅, 曲鑫建. 人诱导多能干细胞分化为心肌细胞关键基因的生物信息学分析[J]. 生命科学研究, 2022, 26(4): 349-360.
TU Si-mei, QU Xin-jian. Bioinformatics Analysis of Key Genes Implicated in Differentiation of Human Induced Pluripotent Stem Cells to Cardiomyocytes. Life Science Research, 2022, 26(4): 349-360.