Abstract：Abstract: CD59 is a glycosylphosphatidylinositol-anchored glycoprotein that consists of 128 amino acids, and is an important member of membrane-bound complement regulatory proteins, which is expressed in blood and a variety of tissues. In this study, transcriptome sequencing data analysis found that the CD59 ex-pression was down-regulated in leukocytes of peripheral blood in patients with early non-small cell lung cancer (NSCLC). On this basis, the protein structure and expression change of CD59 in lung cancer, and the relationship between CD59 expression and the survival of lung cancer patients were analyzed by using databases of Oncomine, GEPIA, UniProt, COMSIC, SWISS-MODEL, STRING and Kaplan-Meier Plotter. Further, the CD59 protein expression levels in the tissues and sera of early lung cancer were analyzed by Western-blot. Oncomine and GEPIA database analysis showed decreased mRNA expression of CD59 in lung cancer tissues, and Western-blot also showed decreased CD59 protein expression levels in the tissues and sera of early lung cancer. Based on COSMIC database analysis, there were three mutated sites (the 8th, 87th, and 128th amino acids) in the CD59 protein of lung cancer patients. The mutated sites at both 8th and 128th amino acids were synonymous mutations, while the amino acid site at tyrosine (Y) 87 was mutated into phenylalanine (F). Meanwhile, the CD59 protein structure was constructed by using the SWISS-MODEL. It was found that tyrosine 87 had hydrogen bonding only with isoleucine (I) 53, and the hydrogen bonding force did not change between phenylalanine 87 and isoleucine 53 in the CD59 mutant when analyzed by SPDBV software, therefore this mutation may not change the protein structure. The protein-protein interaction network analysis showed that CD59 protein was interacted with ten proteins including CD55, C9, C3AR1, C8A, C8B, INS, PLAUR, CD47, ITGAM and CEACAM8. Kaplan-Meier Plotter database analysis revealed that the pa-tients with lower CD59 expression had a shorter survival time. The analysis of CD59 involvement in lung cancer in this study may provide a helpful clue for exploring the mechanism of lung cancer and prognosis eva-luation.
引用本文:
王卫东, 董婧珂, 龚永生, 孙 慧, 王 洁, 金艳霞. 基于生物信息学分析探讨CD59在肺癌中的表达及其临床意义[J]. 生命科学研究, 2022, 26(4): 361-369.
WANG Wei-dong, DONG Jing-ke, GONG Yong-sheng, SUN Hui, WANG Jie, JIN Yan-xia. Analysis of Expression and Clinical Significance of CD59 in Lung Cancer Based on Bioinformatics Databases. Life Science Research, 2022, 26(4): 361-369.
