Abstract：Abstract: Dengue fever (DF) is an acute infectious disease caused by dengue virus (DENV) types 1~4. Antibody-dependent enhancement (ADE) is the main reason of self-limited dengue fever (DF), life-threatening dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS). Different dilutions of anti-DENV2 prM antibody and DENV3 isolated from a patient in Xishuangbanna, Yunnan, were mixed to infect the THP-1 cells. Analysis with real-time quantitative fluorescent PCR demonstrated that the viral load was higher in the THP-1 supernatant fluid when a sub-neutralizing concentration of prM antibody was used. The study on THP-1 cell line lays a foundation for subsequent experiments on ADE of dengue virus infection.
引用本文:
姜黎明, 杨佳佳, 罗 佳, 叶 超, 文送娇, 孙强明. Ⅲ型登革病毒在THP-1细胞上的ADE模型建立[J]. 生命科学研究, 2017, 21(5): 429-432.
JIANG Li-ming, YANG Jia-jia, LUO Jia, YE Chao, WEN Song-jiao, SUN Qiang-ming. Establishment of Dengue Virus 3-ADE Infection Model in THP-1 Cells. Life Science Research, 2017, 21(5): 429-432.
