Abstract:Abstract: In order to investigate the effect of nuclear factor erythroid 2-related factor 2 (Nrf2) on tumor meta-bolism and, consequently, tumor migration, we focused on regulation of lactate dehydrogenase A (LDHA) expression by Nrf2. Herein, the plasmid LDHA-luc containing the firefly luciferase reporter gene driven by human LDHA gene promoter was successfully constructed, and transfected into A549 cells together with Nrf2 expresion vector. After transfection, the dual luciferase reporter gene assay system showed that the LDHA promoter activity in cells transfected with Nrf2 plasmid was significantly higher than that of the control. Meanwhile, the expression level of LDHA in cells transfected with Nrf2 plasmid was also higher, indicating that Nrf2 promoted transcription and expression of LDHA in A549 cells. Up-regulation of LDHA promotes the formation of acidic tumor microenvironment and cell migration. Therefore, successful construction of the plasmid LDHA-luc and demonstration of LDHA gene expression regulation by Nrf2 lay a foundation for further research on how Nrf2 influences tumor metabolism to promote cell migration through LDHA.
引用本文:
曹玉祥, 周兴路, 李祥瑞, 黄 滔, 柯丽霞, 吴志浩. 人源LDHA基因启动子质粒的构建及在肺癌细胞中Nrf2对其转录表达调控和功能分析[J]. 生命科学研究, 2018, 22(1): 36-41.
CAO Yu-xiang, ZHOU Xing-lu, LI Xiang-rui, HUANG Tao, KE Li-xia, WU Zhi-hao. Construction of Human LDHA Gene Promoter Plasmid and Analysis of Its Transcriptional Regulation and Function by Nrf2 in Lung Cancer Cells. Life Science Research, 2018, 22(1): 36-41.