Abstract��Abstract: To optimize biogas production efficiency and investigate the microbial diversity of rural biogas tank sludge, the analysis technique of DGGE for 16S rDNA V3 of microbe in the rural biogas tank was successfully established by the optimized PCR-DGGE. And then the bacterial and archaeal diversity with the change of sludge depth of biogas tank were studied using DGGE. At the same time, the mcr A gene library of sludge samples were constructed, based on this, the diversities of mcr A gene of rural biogas tank sludge were investigated using RFLP. The rural biogas tank sludge contains rich microbe, the sludge depth had less influence on the distribution of the bacterial community, while the archaea communities had obvious distinction with the change of the sludge depth. The results showed the biogas tank sludge had abundant methane producing bacteria, which had similar functions or produce similar metabolic substances. These results provide a basis for further optimizing the community structure and screening functional genes of methane synthesis.
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LI Sang, ZHANG Lin, QIU Yi-Lan, LIU Sheng-Zi, SUN Yi-Bing, YAN Heng-Mei, GUO Xiang-Rong, LIU Ru-Shi. Study on Microbial Diversities in Rural Biogas Digesters. Life Science Research, 2015, 19(4): 321-327.