Abstract:Abstract: Amyloid β-protein precursor (APP) is a major molecule inducing neurodegeneration in diabetic patients. To explore the effects of high-glucose cultivation on the injury of primary hippocampal neurons and the expression of APP, the primary hippocampal neurons were separated from the embryonic day 16 (E16) SD rats and then cultured in the presence of 25 mmol/L, 50 mmol/L, 75 mmol/L and 100 mmol/L glucose, respectively. The cell morphology was observed with an inverted microscope and the expression of APP protein was detected by Western-blot. The results indicated that, following exposure to high concentrations of glucose, the axons of hippocampal neurons became swelling and the synapses gradually grew shorter, while the expression of APP increased. The results suggested that APP might play important roles in high glucose-induced hippocampal neuron damage and that glycemic control would be potentially beneficial for the protection of neuronal cells.
引用本文:
邹译娴, 汤参娥, 赵 师, 刘 芬. 高糖对海马神经元形态破坏和APP蛋白含量的影响[J]. 生命科学研究, 2017, 21(6): 519-522.
ZOU Yi-xian, TANG Can-e, ZHAO Shi, LIU Fen. Effects of High Glucose on the Cellular Structures of Hippocampal Neurons and Expression of APP. Life Science Research, 2017, 21(6): 519-522.