Abstract：Abstract: In order to study the effect of CYP4A inhibitor HET0016 on the tension of isolated mouse aorta, male C57BL/6J mice were put to death by dislocation and aortic vascular rings of 3~4 mm long were cut and fixed in the bath of the microvessel measuring instrument, respectively with high potassium solution (KCl 60 mmol/L) and phenylephrine (Phe 1 μmol/L) for vascular function detection. It was found that both made isolated aortic rings produce sustained contraction. Then the cumulative medication was used to observe the effects of different concentrations of HET0016 on tension of aorta rings in 1 μmol/L Phe treatment group, 60 mmol/L KCl treatment group, 1 μmol/L Phe treatment group after incubation with eNOS inhibitor L-NAME (100 μmol/L) or L-calcium channel blocker nifedipine (1 μmol/L), or the both, and to explore its possible mechanism. The results showed that HET0016 with high concentration could relax high potassium and Phe precontracted endothelium-intact aortic rings; for endothelium-intact aortic rings precontracted by Phe after L-NAME incubation alone, only the high concentration of HET0016 had significantly relaxing effect; for intact aortic rings precontracted by Phe and incubated with nifedipine alone or with both L-NAME and nifedipine, the relaxing effect of HET0016 was obviously concentration-dependent. These results suggest that the relaxing effect of HET0016 is of multi-channel, partly endothelium-dependent, but is not mainly through L-voltage-gated calcium channels, and that only high concentration affects the L-voltage-gated calcium channels.
引用本文:
赵永攀, 李 晋, 邓春玉, 邝素娟, 严华成, 石 磊, 李 健, 赵树进. CYP4A抑制剂HET0016对小鼠离体主动脉血管张力的影响[J]. 生命科学研究, 2016, 20(6): 521-524.
ZHAO Yong-Pan, LI Jin, DENG Chun-Yu, KUANG Su-Juan, YAN Hua-Cheng, SHI Lei, LI Jian, ZHAO Shu-Jin. The Effects of HET0016, the Inhibitor of CYP4A, on Tension of Isolated Aortic Rings of Mice. Life Science Research, 2016, 20(6): 521-524.
2016, Vol. 20 
