Abstract：Abstract: The leaf regeneration in vitro is an important way of rapid propagation and genetic transformation of rabbiteye blueberry. The regeneration pathway for leaf explants of rabbiteye blueberry were firstly clarified by tissue culture and paraffin section techniques，in which the tube plantlet leaves of rabbiteye blueberry “Premier” were used as explants. The results showed that the adventitious buds on the leaf explants could basically be formed within 30 d in vitro culture, going through the processes of occurrence, development and formation. The adventitious buds were regenerated from different tissues of leaf explants of rabbiteye blueberry by direct regeneration way. The meristems originated from the epidermal cells, the parenchyma ones of vascular tissues, and the surrounding ones, which formed meristematic cells by cellular division and differentiation and then formed buds and shoots directly. What’s more, the adventitious buds were successively produced with single buds or multiple shoot clumps on leaf explants, which indicates that the formation of adventitious buds had some particular space-time characteristics.
引用本文:
邱义兰, 陈冰心, 廖丽娟, 刘胜姿, 刘如石. 兔眼蓝莓离体叶片再生组织细胞学观察[J]. 生命科学研究, 2016, 20(6): 516-520.
QIU Yi-Lan, CHEN Bing-Xin, LIAO Li-Juan, LIU Sheng-Zi, LIU Ru-Shi. Histological and Cellular Observations of Regeneration from Leaf Explants of Rabbiteye Blueberry (Vaccinium ashei Reade) in vitro. Life Science Research, 2016, 20(6): 516-520.
2016, Vol. 20 
