Abstract:Abstract: Human mitochondrial transcription termination factor 4 (MTERF4) gene belongs to MTERF gene superfamily. MTERF4 protein plays an important role in the mitochondrial ribosome biogenesis and the regulation of mitochondrial translation. However, the potential regulation mechanism of human MTERF4 gene expression is still undiscovered. In order to investigate the regulation mechanism of human MTERF4 gene promoter, online bioinformatics software and phylogenetic foot-printing methods were used to analyze the promoter distribution, transcription factors and their binding sites, CpG islands of human MTERF4 gene. The results showed that there are at least three promoters in the 5′ uncoding region of human MTERF4 gene. The core promoter of human MTERF4 gene is located between 1 799 bp and 2 368 bp, which plays a key role in the transcription of MTERF4. In addition, 18 transcription factor binding sites were found in conserved promoter region of human and mouse homologous MTERF4 genes by P-Match 1.0 protocol. A 558 bp CpG island was found in human MTERF4 gene promoter region. Bioinformatics analysis of human MTERF4 gene promoter can improve the efficiency of promoter research, and provide significant information for the further experimental research of gene promoter function.
引用本文:
熊 伟, 杨勇琴, 余 敏, 孙美涛, 左绍远, 张海洋. 人MTERF4基因启动子的生物信息学研究[J]. 生命科学研究, 2016, 20(6): 510-515.
XIONG Wei, YANG Yong-Qin, YU Min, SUN Mei-Tao, ZUO Shao-Yuan, ZHANG Hai-Yang. Bioinformatics Study of Human MTERF4 Gene Promoter Region. Life Science Research, 2016, 20(6): 510-515.