Abstract:Abstract: Previous study showed that the expression level of circadian associated repressor of transcription a (ciarta) gene, which is also known as c1orf51, is commonly induced in eight tissues of zebrafish under cold stress. To identify the core cold-regulatory region of ciarta promoter, five fragments of different lengths(-1 992~+100 bp, -1 354~+100 bp, -881~+100 bp, -634~+100 bp and -247~+100 bp) were cloned into pGL4.10, which were transfected into zebrafish-derived ZF4 cells. The cold-regulatory abilities of these 5 fragments were quantified by examining the expression changes of luciferase at a cold temperature. The results showed that all of the five fragments could significantly induce the gene expression at the cold temperature. Of these 5 fragments, one fragment (-881~+100 bp) showed the highest cold-induced ability and increased the downstream gene expression up to 5.61±0.53 fold. Compared with the fragment (-634~+100 bp), the fragment (-881~+100 bp) was found to contain a known cold-induced cis-element (BCL6 binding site). The finding verifies the cold-induced ability of BCL6 binding site. The cold-induced key promoter region of ciarta can be used as a tool to induce the expression of the gene at low temperature, which is helpful for the related research.
引用本文:
张 东, 胡 鹏, 陈良标. 斑马鱼ciarta基因启动子低温诱导核心区域的鉴定[J]. 生命科学研究, 2016, 20(6): 480-485.
ZHANG Dong, HU Peng, CHEN Liang-Biao. Identification of Cold-induced Key Promoter Region of ciarta Gene in Zebrafish. Life Science Research, 2016, 20(6): 480-485.