Abstract：Abstract：Long noncoding RNAs (lncRNAs) play an important role in the regulation of cellular function. It is known that cerebral ischemia/reperfusion injury induces many changes of lncRNAs expression, but the regulation mechanism of lncRNAs in ischemic stroke remains largely unknown. Here, the changes of lncRNAs and mRNAs in brain tissue after ischemia/reperfusion injury were analyzed through downloading gene chip data from GEO. A total of 27 lncRNAs and 408 mRNAs were found to be up-regulated, while 9 lncRNAs and 31 mRNAs were down-regulated. Meanwhile, gene ontology analysis and pathway analysis indicated that dysregulated transcripts were enriched in immune response, inflammatory response, cell apoptosis and corresponding pathways. Additionally, based on lncRNAs target prediction programs and lncRNAs-mRNAs co-expression networks which combine the analysis of RNA sequence and gene expression, dysregulated lncRNAs were predicted to regulate cell apoptosis, immune and inflammatory responses. And potential targets and regulation mechanism of some lncRNAs were inferred, such as lincRNA (chr19:5771425-5848475_F), lincRNA (chr1:137503023-137625604_R), lincRNA (chr14:21029450-21049451_F) and so on. Taken together, these findings show that lncRNAs are involved in the regulation of cerebral ischemia/reperfusion injury to a great extent, which provide potential research directions for injury mechanism and therapeutic strategy in ischemic stroke.
引用本文:
冯维熙, 曹 新. 基于目标基因芯片数据预测lncRNAs调控脑缺血再灌注损伤后的作用效应[J]. 生命科学研究, 2016, 20(3): 235-242.
FENG Wei-Xi, CAO Xin. Role of Long Non-coding RNAs in the Regulation of Post-cerebral Ischemia/Reperfusion Injury Based on Analysis of Target Gene Chip Data . Life Science Research, 2016, 20(3): 235-242.
2016, Vol. 20 
