Abstract:Abstract: Selection of stable reference genes is a prerequisite for accurate application of real-time quantitative PCR (qRT-PCR) analysis in Siniperca chuatsi. Seven reference genes, including RPL13, RPL19, EF1a, RPL13a, B2M, hprt1 and rps29 were chosen to analyze expression stability by qRT-PCR in different adult tissues and developmental stages. The results showed that B2M and RPL13a were the most stable reference genes in different adult tissues. However, in different embryonic developmental stages, EF1a and B2M were the most stable reference genes, and in different post-embryonic developmental stages, EF1a and RPL13a were the most stable genes. On the basis of the normalization factor Vn/n+1, the optimum number of reference genes was two either in different adult tissues or in different developmental stages. The present study provided a useful method of choosing suitable reference genes in gene expression analysis in Siniperca chuatsi.
引用本文:
李 迪, 吴 萍, 何美凤, 李 伟, 肖调义, 褚武英. qRT-PCR分析鳜鱼内参基因的筛选[J]. 生命科学研究, 2016, 20(3): 214-217.
LI Di, WU Ping, HE Mei-Feng, LI Wei, XIAO Diao-Yi, CHU Wu-Ying. Screening of Reference Genes in Siniperca chuatsi for qRT-PCR Analysis. Life Science Research, 2016, 20(3): 214-217.