Abstract：Abstract: Western-blot and immunohistochemical staining were used to detect the expression of c-Jun N-terminal kinase (JNK) and extracellular regulated protein kinase (ERK) in gonad tissues of different ploidy crucian carps. It was found that JNK and ERK had high-level expression in fish gonad tissues and that no significant difference existed in the expression level of JNK or ERK between the ovary and testis as well as between diploid gynogenetic progeny and different ploidy level fishes. Expression of P-JNK in the gonad tissues of diploid gynogenetic progeny was much higher than that in triploid or tetraploid ovary. Expression of both JNK and ERK was detected positive in the spermatogonium of early gonad tissues in diploid gynogenetic progeny of different ages. JNK showed more strongly positive reaction in 10-month-old fish than in 6- and 8-month-old fish, while the positive reactions of ERK in 8-and 10-month-old fish were weaker than that in 6-month-old fish. The results indicated that JNK pathway might play an important role in regulation of unreduced gamete formation in diploid gynogenetic progeny.
引用本文:
王姿力, 骆瑜蓉, 罗仲文, 王 静, 肖亚梅. MAPK在不同生殖特性鲫鲤杂交鱼性腺组织中的表达分析[J]. 生命科学研究, 2016, 20(2): 95-101.
WANG Zi-Li, LUO Yu-Rong, LUO Zhong-Wen, WANG Jing, XIAO Ya-Mei. Expression of MAPK in the Gonadal Tissue of Different Reproductive Characteristics of the Hybrid Crucian Carp. Life Science Research, 2016, 20(2): 95-101.
2016, Vol. 20 
