Abstract:Abstract: Galectin-3 (Gal-3) is a multi-functional protein involved in many biological processes such as cell proliferation, differentiation, apoptosis, angiogenesis, oncogenesis and metastasis. In previous studies, gal-3 cDNA diversity was indicated in some amphibian species. To clarify if such diversity exists in mammals, the cloning and sequencing of gal-3 open reading frame (ORF) was performed from 3 individuals’ liver first strand cDNAs of New Zealand rabbit Oryctolagus cuniculus. As the result, 5 single nucleotide polymorphism (SNP) were observed resulting in 11 different clones, one of which is the same as that deposited in GenBank, while the others are new and have never been reported. In fact, 6 different clones were obtained from both the first and the second individuals, and 2 were ohtained from the third one. All these analysis indicated gal-3 cDNA diversity at individual level in the liver of O. cuniculus.
引用本文:
方恩浩, 张珊珊, 刘倩芸, 周建钟, 杨仙玉. 兔半乳糖凝聚素-3 cDNA分子多样性分析[J]. 生命科学研究, 2015, 19(6): 512-515.
FANG 恩Hao, ZHANG Shan-Shan, LIU Qian-Yun, ZHOU Jian-Zhong, YANG Xian-Yu. Galectin-3 cDNA Diversity in Oryctolagus cuniculus. Life Science Research, 2015, 19(6): 512-515.