Abstract:Abstract: Pinctada martensii is a major economic shellfish for sea pearls. Due to the diseases anually, a large of P. martensii died every year and resulting in a huge economic losses on China’s pearl industry. The immune system and the mechanism of P. martensii against pathogenic bacteria is not clear so far. Antimicrobial peptide is one of the most important immue molecular for shellfish, however, little sequence and characterization of antimicrobial peptides was reported from P. martensii. In order to understand the protein composition and molecular diversity of P. martensii antimicrobial peptide, multi-step high performance liquid chromatogram (HPLC) combined mass spectrometry were performed to exploring the peptides with antimicrobial activity. 5 components with significant anti-bacteria activity were isolated from the serum of P. martensii and 3 of them were homogeneous with molecular mass of around 5 kD proved by MALDI-TOF. Furthermore, the components caused significant morphological alterations in E. coli as shown by transmission electron microscopy (TEM). These results indicated that the peptides isolated from P. martensii serum with molecular mass of 5 kD were involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria.
引用本文:
朱家萍, 雷倩楠, 梁海鹰. 马氏珠母贝血清抗菌肽的初步分析[J]. 生命科学研究, 2015, 19(5): 397-401.
ZHU Jia-Ping, LEI Qian-Nan, LIANG Hai-Ying. Analysis of Antimicrobial Peptides from Serum of Pinctada martensii. Life Science Research, 2015, 19(5): 397-401.