Abstract:Abstract: To investigate the relationship between methylation of amyloid precursor protein (APP) gene and coronary heart disease (CHD), the APP methylation levels of 538 CHD patients and 453 normal controls were detected using the quantitative methylation-specific PCR (qMSP). The results showed that the age, the numbers of males, smokers and diabetic patients were significantly higher in CHD patients than in controls (age, P=8.0E-08; males, P=7.0E-07; smokers, P=0.001; diabetes, P=0.019). Albumin levels were significantly lower in the CHD group than in the control group (P=0.001), while AST, ALP, and γ-GT levels were significantly higher in the CHD group than in control group (AST, P=3.0E-04; ALP, P=0.001; γ-GT, P=0.018). In both total and male samples, the methylation levels of the APP gene were found to be significantly higher in the CHD group than in the control group (total samples, P=0.026; males, P=0.025). In non-smoking CHD patients, the APP methylation level was positively related to the degree of stenosis (r=0.076, P=0.046). In both total CHD patients with hypertension and CHD male patients with hypertension, APP methylation levels were found to be positively related to the degree of stenosis (total samples, r=0.096, P=0.029; males, r=0.135, P=0.019). After age stratification, it was found that the APP methylation level was positively related to stenosis in CHD male patients with hypertension aged ≥63 years (r=0.219, P=0.020). In non-smoking or non-hypertensive CHD patients aged <63 years, APP methylation levels were found to be negatively correlated with stenosis (non-smoking, r=-0.223, P=0.008; non-hypertension, r=-0.216, P=0.010). In CHD male patients, a positive correlation was found between the APP methylation level and the age (r=0.163, P=0.001). In CHD female pa-tients, an inverse correlation was observed between the APP methylation level and the age (r=-0.192, P=0.015). In normal male controls, the APP methylation level was inversely correlated with the age (r=-0.203, P=0.001). In normal female controls, APP methylation level was inversely correlated with ApoB, albumin and ALT levels (r=-0.160, P=0.028; r=-0.151, P=0.036; r=-0.163, P=0.024, respectively). In the normal group, the APP methylation level was positively correlated with Lp(a) level (r=0.108, P=0.031). In conclusion, the above results showed that the increased methylation level of APP gene might be related to the risk of CHD in males.
引用本文:
季慧慧, 郑中华, 段世伟. APP基因启动子区甲基化与冠心病相关性的研究[J]. 生命科学研究, 2019, 23(3): 173-182.
JI Hui-hui, ZHENG Zhong-hua, DUAN Shi-wei. Study on the Correlation Between Methylation of APP Gene Promoter Region and Coronary Heart Disease. Life Science Research, 2019, 23(3): 173-182.