Abstract: Abstract: In mammals, the TRAF family member-associated NF-κB activator (TANK) is involved in the regulation of the nuclear factor-κB (NF-κB) signaling pathway. Recent studies have showed that TANK plays an important role in the antiviral innate immune activation of black carp. However, in one of the most widely used animal model zebrafish, there is no report on its function. Herein, zebrafish (Danio rerio) TANK (DrTANK) was cloned and characterized. The DrTANK comprises 1 047 nucleotides and the predicted protein contains 349 amino acids. The DrTANK migrated to approximately the 50 kD position in immunoblotting assay. The DrTANK was identified as a cytoplasmic protein by immunofluorescence staining. DrTANK could not induce the activity of interferon (IFN) promoter in luciferase reporter assay in EPC cells; however, the IFN-activation ability of DrTANK was obviously enhanced when the cells were treated with poly (I:C) or grass carp reovirus (GCRV). In summary, our data suggest that the DrTANK may play an important role in zebrafish antiviral innate immune response. This result benefits the functional study of the DrTANK in future.