Abstract：Abstract: Ornithine decarboxylase antienzyme inhibitor-1 (OAZI-1) is an important protein factor involved in the regulation of polyamine metabolism. Studies had found that melanoma cells with OAZI-1 overexpression could be effectively identified and engulfed in vitro by antigen-presenting cells, and this suggested a potential value of OAZI-1 in tumor immunotherapy. In order to explore the effect of overexpression of OAZI-1 on growth of B16-F1 melanoma cells in mice, the B16-F1 cells with OAZI-1-overexpression (B16/OAZI-1) were inoculated into the mice. The results showed that the resulted tumor mass increased normally within first 12 days and then began to shrink gradually. At the day 24, mean tumor volume in the mice inoculated with control tumor cells was 326±309 mm3 but only 36±25 mm3 in the mice inoculated with B16/OAZI-1 cells. To explore the underlying mechanism for this phenomenon, OAZI-1-mediated immune effects were determined. The results showed: 1) the spleen cells of mice inoculated with B16/OAZI-1 had enhanced cytotoxic activity against B16-F1 tumor cells; 2) proliferation of the splenocytes from B16/OAZI-1-inoculated mice was more efficiently stimulated by the lysate from B16-F1 cells; 3) the splenocytes from the B16/OAZI-1-inoculated mice exhibited elevated interferon-γ (INF-γ) secretion; 4) when the mice were inoculated with B16/OAZI-1 followed by re-challenge with live B16-F1 cells 30 days later, a decreased tumor growth and prolonged mouse survival time were observed. These results suggest that overexpression of OAZI-1 can inhibit the growth of B16-F1 melanoma cells in mice. The underlying mechanism may be related to the ability of OAZI-1 in promoting tumor antigen presentation and inducing anti-tumor immune response.
引用本文:
杨建林, 曹春雨, 秦 烨, 吴红艳, 王艳林. 鸟氨酸脱羧酶抗酶抑制因子-1高表达抑制黑素瘤细胞在小鼠体内的生长[J]. 生命科学研究, 2017, 21(4): 343-348.
YANG Jian-lin, CAO Chun-yu, QIN Ye, WU Hong-yan, WANG Yan-lin. Overexpression of Ornithine Decarboxylase Antienzyme Inhibitor-1 Inhibits Growth of Melanoma Cells in Mice. Life Science Research, 2017, 21(4): 343-348.
