Abstract��Abstract: To construct a reporter vector regulated by the hMTERF3 promoter to determine the effect of DNA methylation on the promoter activity of hMTERF3 gene, PCR amplification was performed to obtain seven potential hMTERF3 promoter fragments, which were then cloned into the vector to obtain recombinant constructs. Promoter activities of different hMTERF3 fragments in cancer cells were detected by luciferase assay to identify the potential promoter area. The activity of the hMTERF3 promoter with or without treatment of methylase M. Sss��was also evaluated by luciferase assay. It showed that three fragments (P523, P866 and P932), all of which contained the P523 sequence, had stronger promoter activity than the pGL6 control. Their promoter activities decreased significantly after methylase treatment (P<0.05). A luciferase reporter gene system containing the hMTERF3 promoter was successfully constructed. P523 is the potential core area of the hMTERF3 promoter. These results provide a basis for studying the epigenetic mechanism of hMTERF3 gene expression in hepatoma cells.
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�� ΰ, ������, ÷ ��, �ԼӼ�, ������, ����Զ, ������. �ΰ�ϸ��hMTERF3���������ӱ������Ĺ���������DNA������Ӱ��[J]. ������ѧ�о�, 2017, 21(4): 289-294.
XIONG Wei, SUN Mei-tao, MEI Wen, ZI Jia-ji, YANG Yong-qin, ZUO Shao-yuan, ZHANG Xiao-juan. Construction of a Reporter Vector Regulated by hMTERF3 Promoter and Effect of DNA Methylation on hMTERF3 Promoter Activity in Hepatoma Cells. Life Science Research, 2017, 21(4): 289-294.
