Abstract��Abstract: Small interfering RNAs (siRNAs) targeting ICP27 gene for herpes simplex virus 1 (HSV-1) and other highly conserved sequences for herpesviruses were designed and detected for their abilities to inhibit herpesvirus replication in vitro. Virus titer assay, real-time PCR and cytopathic effect (CPE) experiment were used to observe their inhibition effect in Vero cells challenged with HSV-1 by three small hairpin RNAs (shRNA-1, shRNA-2 and shRNA-3). The results suggested shRNA-2 (target sequence from 815 position) and shRNA-3 (target sequence from 1 367 position) could depress the replication of HSV-1. Especially, virus replication was obviously inhibited by shRNA-3 in the virus titer assay, and the inhibition ratio was 81 times comparing with Negative group. At the same time, the mRNA expression level of ICP27 gene could be decreased by shRNA-3. It indicated that shRNA-3 could effectively suppress the replication of HSV-1 and be used as a supplementary therapy for HSV-1 infectious diseases. The corresponding target sequence of shRNA-3 could therefore be used as a new target for anti-HSV-1 drugs.
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������, �� ��, �� ��, ȫ��÷, �� ΰ. �����Ը���ICP27�����siRNA��HSV-1�������Ƶ�Ӱ��[J]. ������ѧ�о�, 2017, 21(2): 130-135.
WANG Xiao-Ting, DONG Hui, WANG Jun, QUAN Jin-Mei, ZHANG Wei. Inhibition of HSV-1 Virus Production by siRNAs Specific for ICP27 Gene. Life Science Research, 2017, 21(2): 130-135.
2017, Vol. 21 
