Abstract��Abstract: To explore the effects of ischemic preconditioning on myocardial ischemia-reperfusion injury, the myocardial ischemia-perfusion model was established in C57/B6 male mice aged 6~8 weeks. Thirty-six mice were randomly divided into three groups: sham group (Sham, n=12), ischemia-reperfusion group (I/R, n=12) and ischemic preconditioning group (Ipost, n=12). The hematoxylin-eosin staining (HE), TUNEL, immunohistochemical staining, and Western-blot were used to detect the changes in myocardial tissues from three groups. Compared with the Sham group, the histopathological changes, including myocardial tissue edema and inflammatory cell infiltration, were obviously serious in the I/R group. However, Ipost group was improved significantly compared with the I/R group. TUNEL results showed that the myocardial apoptosis rate in Ipost group was significantly lower than that in I/R group (P<0.01), though higher than that in the Sham group. In Ipost group, the number of capillaries on the edge of myocardial infarction area was significantly higher than that in I/R group (P<0.01). Western-blot results indicated that the expression of PTEN protein in the myocardial tissue in Ipost group was down-regulated, whereas the AMPK phosphorylation and LC3I/II expressions were increased. Taken together, ischemic preconditioning may have protective effects on I/R injury, and could reduce the infarct area. The protective mechanism of Ipost may be related to reduction of myocardial cell apoptosis, down-regulation of PTEN, enhancement of the phosphorylation level of AMPK and enhancement of myocardial cell autophagy in ischemic infarcted myocardium after pretreatment.
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LI Jia-Ni, LI Jia-Xin, YAN Jin-Peng, YE Yan-Qiong, LUO Ling, YANG Bin, ZHAN Ji-Chun, SU Qi-Li, ZHUANG Jian, CHEN Ji-Mei, ZHU Ping, ZHAO Ming-Yi. Effects of Ischemic Preconditioning on Myocardial Ischemia-reperfusion Injury in Mice. Life Science Research, 2017, 21(2): 111-116.
2017, Vol. 21 
