Abstract：Abstract: Exploring the methods for primary culture of atrial myocytes from patients with atrial fibrillation (AF) can provide practical methods for research about AF. Here, the left atrial appendage specimens were obtained from patients with AF during cardiac surgery. Then, primary atrial myocytes, which were isolated from the specimens by collagenase digestion method, were identified through immunohistochemistry and analyzed simultaneously. In contrast with some animal experiments, low expressions of connexin40 (Cx40) and Kv1.5 channel protein were found in AF patients. The results showed that studying atrial myocytes of AF patients will be more reliable. Meanwhile, atrial myocyte culture from AF patients is the basis for research on the pathogenesis of AF.
引用本文:
卞誉豪, 张 飞. 房颤患者心房肌细胞的原代培养与应用[J]. 生命科学研究, 2017, 21(1): 51-54.
BIAN Yu-Hao, ZHANG Fei. Primary Culture and Application of Atrial Myocytes from Patients with Atrial Fibrillation. Life Science Research, 2017, 21(1): 51-54.
2017, Vol. 21 
