Abstract��Abstract: To establish glucagon-like peptide-1 receptor (GLP-1R) cellular model for screening GLP-1R ligands. A eukaryotic expression vector pCMV6-GLP-1R-GFP was constructed and transfected into U2OS cells. A stable expressing GLP-1R-GFP U2OS cell line was established and the expression of GLP-1 receptor was confirmed by Western-blot with a GLP-1 specific antibody. The activities of expressed GLP-1R-GFP were investigated with the cellular redistribution of green fluorescence after stimulation with GLP-1 analogs. The sensitivity and specificity of this cell line was qualified and the results showed that the cell line was suitable for GLP-1 receptor ligand screening.
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LI Ming-Rui, JI Xiu-Ling, ZHANG Min, ZHANG Kuan-Ren, LIU Dong-Bo, WEI Yun-Lin. Establishment of a GFP Fluorescence Labeled GLP-1 Receptor Cell Line. Life Science Research, 2015, 19(6): 501-504.