Abstract:Abstract: Long non-protein coding gene (lncRNA) in C. elegans gains great mania in recent years. linc-5 is a lncRNA which has high expression level in embryo and early larva according to the RNA-seq data, suggesting its importance during the development of C. elegans. Based on two species C. elegans and C. briggsae, expression vector of promoter and fluorescent protein (mCherry) fusion was constructed as reporter gene. Reporter gene was integrated to genome using gene bombardment and got homozygous transgene strains of C. elegans and C. briggsae. Then the expression pattern was compared in L1 larva stage using the well-established technique of analysis in single cell resolution, and it showed strong conservation between the two species. Through the genome editing tools CRISPR/Cas9, linc-5 was knocked out successfully, which provided infrastructure for further study on lncRNA.
引用本文:
于恩超, 陈思雨, 赵晓彤, 刘卫红, 赵 娣, 李勇斌, 刘 晓. 一个线虫长非编码RNA的单细胞精度表达谱分析以及基因敲除研究[J]. 生命科学研究, 2015, 19(6): 484-490.
YU 恩Chao, CHEN Si-Yu, ZHAO Xiao-Tong, LIU Wei-Hong, ZHAO Di, LI Yong-Bin, LIU Xiao. Analysis of Expression Pattern in Single Cell Resolution of an lncRNA and Gene Knock-out in C. elegans. Life Science Research, 2015, 19(6): 484-490.