Abstract：Abstract: To observe the effects of polypeptide K-YFAE on the proliferation and hypoxia tolerance of H9C2 cardiomyocytes, H9C2 cardiomyocytes were cultured in vitro and divided into control and polypeptide groups. In the control group, only 10% dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS) were used, and in the polypeptide groups, different concentrations (10 μmol/L, 20 μmol/L and 50 μmol/L) of polypeptide K-YFAE were added. After 7 days, CCK-8, flow cytometry and qPCR were conducted to observe the effects of K-YFAE on the proliferation of H9C2 cardiomyocytes. After hypoxia treatment in three-gas incubator, the cells were grouped, for apoptosis detection by both TUNEL assay and flow cytometry, and for apoptosis-related genes by qPCR. The results showed that, compared with the control group, the intervention of K-YFAE promoted H9C2 cardiomyocytes to enter the proliferation phase and decreased their apoptosis, which means that K-YFAE may not only promote the proliferation of H9C2 cardiomyocytes, but also increase their hypoxia tolerance.
引用本文:
王靠山, 朱 莉. 多肽K-YFAE对H9C2心肌细胞增殖及缺氧耐受的影响[J]. 生命科学研究, 2022, 26(1): 47-53.
WANG Kao-shan, ZHU Li. Effects of Polypeptide K-YFAE on Proliferation and Hypoxia Tolerance of H9C2 Cardiomyocytes. Life Science Research, 2022, 26(1): 47-53.
