Abstract：Abstract: Understanding the effect and the molecular mechanism of rapamycin-induced autophagy on mi-gration of cervical cancer SiHa cells will provide an experimental basis for finding new targets and new methods for cervical cancer therapy. The autophagy induced by rapamycin in SiHa cells was observed by de-tecting the changes of microtubule-associated protein light chain 3Ⅱ (LC3Ⅱ)/LC3Ⅰ after the cells were treated with the maximum concentration of rapamycin which did not affect the viability of the cells, and then the migration ability of SiHa cells was observed by wound healing assay. The levels of matrix metallopro-teinase 2 (MMP2) in SiHa cells were detected by Western-blot. The results showed that, after SiHa cells were treated with 20 nmol/L rapamycin, the ratio of LC3Ⅱ/LC3Ⅰ in cells was 291.282%±28.463% as much as that in the control cells (P<0.05), indicating that autophagy was induced successfully in SiHa cells. The mi-gration rate was 29.886%±1.807% in SiHa cells treated with rapamycin, significantly lower than that (38.914%±1.422%) in the control cells (P<0.05), and the relative level of MMP2 in SiHa cells treated with rapamycin was significantly decreased, which was 61.310%±17.843% as much as that in the control cells (P<0.05). The results suggest that rapamycin-induced autophagy may inhibit the migration of SiHa cells through the MMP2 pathway.
引用本文:
郭宇微, 王樱槥, 解亦航, 赵春艳. 雷帕霉素诱导SiHa细胞自噬并通过MMP2途径抑制细胞迁移[J]. 生命科学研究, 2022, 26(1): 34-38.
GUO Yu-wei, WANG Ying-hui, XIE Yi-hang, ZHAO Chun-yan. Rapamycin Induces Autophagy and Inhibits Cell Migration via MMP2 Pathway in SiHa Cells. Life Science Research, 2022, 26(1): 34-38.
