Abstract：Abstract: To explore the clinical significance of KCTD10 in hepatocellular carcinoma (HCC), the protein and mRNA levels of KCTD10 in normal and HCC tissues, as well as different HCC cell lines, were analyzed by UALCAN database and with methods of immunohistochemistry (IHC), quantitative real-time PCR and Wes-tern-blot. 3,5-Diethoxycarbonyl-1,4-dihydrocollidine (DDC) diet was used to induce a mouse model of liver injury, and the expression of KCTD10 in the liver was detected by IHC. The results showed that KCTD10 expression was significantly up-regulated in HCC tissues compared with paracancerous tissues. Moreover, its expression was positively correlated with the malignant grades of HCC, and was found to increase in the cy-toplasm but relatively decrease in the nucleus of HCC tissues. In Hep3B, Huh7, and MHCC97H cell lines, both mRNA and protein levels of KCTD10 were increased, and the highest expression level of KCTD10 was detected in the cytoplasm of MHCC97H cells. The protein level of KCTD10 was also decreased in the nu-cleus of injured mouse liver tissues. Together, KCTD10 expression has a positive correlation with the occur-rence and development of HCC. The expression level and location change of KCTD10 in HCC cells may provide support for early clinical diagnosis of liver cancer.
引用本文:
肖轶卉, 樊安方, 王道远, 张浩睿, 任凯群. 人肝癌组织及小鼠肝损伤组织中KCTD10的表达分析研究[J]. 生命科学研究, 2022, 26(1): 26-33.
XIAO Yi-hui, FAN An-fang, WANG Dao-yuan, ZHANG Hao-rui, REN Kai-qun. Expression of KCTD10 in Human Hepatocellular Carcinoma and Injured Mouse Liver Tissues. Life Science Research, 2022, 26(1): 26-33.
