Abstract:Abstract: In order to explore the effect of miR-365 on the viability and proliferation of gastric cancer cells and its mechanism of action, RT-qPCR technology was used to detect the endogenous expression levels of miR-365 in gastric cancer cells (AGS and MGC80-3) and normal gastric epithelial cells (GES-1). At the same time, the endogenous expression levels of miR-365 in gastric cancer and adjacent tissues in clinical samples were detected. CCK-8 was used to detect cell proliferation, RT-qPCR and Western-blot were used to detect the mRNA and protein levels of cyclin-dependent kinase 4 (CDK4), CDK6, cyclinD1 and FoxO1, and the luciferase reporter experiment was used to verify the binding ability of miR-365 and FoxO1 3′-UTR. The experimental results showed that, compared with normal gastric epithelial cells, the two gastric cancer cells showed down-regulated expression of miR-365. After miR-365 mimics were transfected, the proliferation activity of gastric cancer cells was decreased, and the mRNA and protein levels of cyclinD1, CDK4, CDK6 and FoxO1 were down-regulated. miR-365 could bind to the 3′-UTR of FoxO1, inhibiting the expression of FoxO1 protein. Compared with adjacent tissues, cancer tissues showed reduced expression of miR-365. The above results indicate that miR-365 could inhibit FoxO1 protein expression by targeting the 3′-UTR of FoxO1, thereby inhibiting the proliferation of gastric cancer cells.
引用本文:
陈 路, 胡 爽, 张慧敏, 王 君, 黄 凤, 王志文. miR-365通过靶向FoxO1抑制胃癌细胞的增殖[J]. 生命科学研究, 2021, 25(2): 109-116.
CHEN Lu, HU Shuang, ZHANG Hui-min, WANG Jun, HUANG Feng, WANG Zhi-wen. miR-365 Inhibits Gastric Cancer Cell Proliferation by Targeting FoxO1. Life Science Research, 2021, 25(2): 109-116.