Abstract：Abstract: The devastating disease caused by Papaya ringspot virus(PRSV) is a serious threat to the development of papaya industry. At present, no effective strategies can prevent PRSV. The infection of plant virus depends on the assistance of host factors. Eukaryotic initiation factor 4E(eIF4E) has been found to involve in the interaction of plant and virus, and is a necessary factor for plants infecfed by a variety of RNA viruses. Based on it, a chimeric hairpin RNA(hpRNA) vector containing papaya eIF4E and eIFiso4E fragments was successfully constructed, and had been transferred into papaya mesophyll protoplast. After detected by real-time RT-PCR, the eIF4E and eIFiso4E gene expression were decreased by 49.8% and 67.6%, respectively. The results will help to study the effect of eIF4E family genes on the PRSV infection, and provide theoretical basis for the new strategy of anti-virus by targeting plant genes with RNAi technology.
引用本文:
霍 鹏, 言 普, 沈文涛, 黎小瑛, 周 鹏. 番木瓜eIF4E和eIFiso4E基因嵌合hpRNA载体一步快速构建及沉默效果的研究[J]. 生命科学研究, 2016, 20(1): 50-56.
HUO Peng, YAN Pu, SHEN Wen-Tao, LI Xiao-Ying, ZHOU Peng. One-step Rapid Construction and Gene Silencing Effect of Chimeric Hairpin RNA Vector of Papaya eIF4E and eIFiso4E. Life Science Research, 2016, 20(1): 50-56.
2016, Vol. 20 
