Abstract：Abstract: DNA Methyltransferase 1 (DNMT1) functions as a maintenance enzyme to ensure the methylation patterns to faithfully copy to progeny cells during DNA replication, while DNA methyltransferase 3b (DNMT3b) mainly regulates the initial methylation for developing genomes. The effects of knockdown of Dnmt1 and/or Dnmt3b on the changes of cellular apoptosis and global DNA methylation in mouse embryonic fibroblast cells (MEFs) were investigated using siRNA-mediated RNAi technology. The results demonstrated that the mRNA levels of proliferating cell nucleus antigen (PCNA) decreased significantly in both Dnmt3b single-knockdown cells and Dnmt1+Dnmt3b double-knockdown cells (P<0.05). Moreover, at 48 h post-transfection, the rates of cells undergoing apoptosis in these two groups increased significantly(P<0.05). The global methylation level of Dnmt3b single-knockdown cells dropped 32% (P<0.01), whereas it decreased about 44% in the Dnmt1+Dnmt3b double-knockdown group(P<0.01). These data suggested that DNMT3b played an important role in the maintenance of cellular proliferation and the global methylation states in MEFs.
引用本文:
吴梦华, 王巨龙, 张 宇, 易建明, 于孟飞. RNA干扰Dnmt1和Dnmt3b对小鼠胚胎成纤维细胞凋亡和基因组DNA甲基化水平的影响[J]. 生命科学研究, 2016, 20(1): 1-7.
WU Meng-Hua, WANG Ju-Long, ZHANG Yu, YI Jian-Ming, YU Meng-Fei. The Impact of Knockdown of Dnmt1 and Dnmt3b on the Apoptosis of Mouse Embryonic Fibroblast Cells and Global DNA Methylation Level. Life Science Research, 2016, 20(1): 1-7.
2016, Vol. 20 
