Abstract��Abstract: To explore the key autophagy-related molecules and the biological processes mediated by THP-1 macrophages after Mycobacterium abscesses (MAB) infection, the autophagy-related genes of MAB-infected THP-1 macrophages were collected from GEO and GeneCards databases. Gene Ontology (GO) and Kyoto En-cyclopedia of Genes and Genomes (KEGG) enrichment analysis of the selected genes were performed by the DAVID online tool. Real-time quantitative PCR (qRT-PCR) was used to verify the screened key genes. The Pearson correlations between the key genes and the autophagy marker molecule were analyzed. Bioinformatics analysis indicated that there were 11 genes closely related to autophagy, and the top five with the largest ex-pression differences were Bcl-2/adenovirus E1 B interacting protein 3 (BNIP3), BMP and activin membrane bound inhibitor (BAMBI), microtubule associated protein 1 light chain 3 (MAP1LC3A), Dysferlin (DYSF) and Myozenin1 (MYOZ1). Function prediction showed that, after MAB infection of macrophages, 11 differentially expressed genes were mainly related to processes such as protein binding and autophagy. qRT-PCR verifica-tion results showed that the changes of BNIP3, MAP1LC3A and MYOZ1 in THP-1 macrophages infected with two phenotypes of MAB were consistent with the results of transcriptome sequencing (RNA-seq). The di-fference between RNA-seq and RT-PCR results of BNIP3 was the most significant (P<0.05). Pearson corre-lation analysis showed that BNIP3 had the strongest correlation with MAP1LC3A (P<0.05). Therefore, BNIP3 proved to be the most autophagy-related molecule after MAB infection of THP-1 macrophages. The study may provide an important clue for exploring molecular mechanisms of autophagy in MAB-infected macrophages.
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�� ��, Ф�ķ�, ʯ����, �� ��, ��С��, ��ǰѡ, ���Ӹ�. ŧ��֦�˾���ȾTHP-1����ϸ��������ط��ӵ�ʶ��[J]. ������ѧ�о�, 2022, 26(6): 549-556.
ZHOU Ting, XIAO Si-fang, SHI Guo-min, YU Rong, MA Xiao-hua, ZHOU Qian-xuan, XIANG Yan-gen. Recognition of Autophagy-related Molecules of MAB-infected THP-1 Macrophages . Life Science Research, 2022, 26(6): 549-556.
