Abstract：Abstract: Antimicrobial peptide LL-37 is closely related to the occurrence and development of tumors. Our previous study found that LL-37 can inhibit the malignant proliferation of hepatocellular carcinoma (HCC) cells. To provide more biological basis for the molecular mechanisms of LL-37 inhibiting the occurrence and development of HCC, high-throughput RNA sequencing and bioinformatics methods were used to analyze the differentially expressed genes (DEGs) in HCC cells before and after LL-37 treatment. A total of 753 DEGs were screened out, among which 374 genes were up-regulated and 379 genes were down-regulated. The se-lected DEGs were further enriched by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, and the protein-protein interaction network encoded by DEGs was also construc-ted. Ten potential key genes that might be involved in inhibition of malignant proliferation of HCC cells by LL-37 were screened out. It was found that these genes were all down-regulated and played an important role in the activation of inflammation as well as in the proliferation and migration of tumor cells. Taken to-gether, these results provide data foundation for revealing the role and mechanism of LL-37 in the occur-rence and development of HCC, and provide new ideas for exploring the diagnosis and treatment of HCC.
引用本文:
吕继龙, 李球棣, 佘东阳, 陈 宁, 丁晓慧. 抗菌肽LL-37抑制肝癌细胞恶性增殖的转录组分析[J]. 生命科学研究, 2022, 26(6): 528-537.
L?譈 Ji-long, LI Qiu-di, SHE Dong-yang, CHEN Ning, DING Xiao-hui. Transcriptomic Analysis of Malignant Proliferation of Hepatocellular Carcinoma Cells Inhibited by Antimicrobial Peptide LL-37. Life Science Research, 2022, 26(6): 528-537.
