Abstract:Abstract: Hydrophobins play an important role in the morphology engineering of filamentous fungi. To clarify hydrophobin genes of Aspergillus niger for further research, assumed hydrophobin genes were analyzed based on the cysteine pattern and hydropathy pattern of hydrophobin amino acid sequences. Eight genes were obtained from 20 assumed hydrophobin genes based on gene sequence similarity. The proteins coded by these eight genes had the 8 cysteines pattern which all belong to class I hydrophobin. While only two hydrophobins had hydropathy pattern of class I, and the other 6 were considered as intermediate type of hydrophobin according to their hydropathy patterns. Finally, the similarity of amino acid sequences of these 8 hydrophobins was 0.5%. The clarification of assumed hydrophobin genes builds a solid base for hydrophobin gene cloning and knockout to confirm their function in fungal spore aggregation.
引用本文:
张 燕, 许 可, 张建国. 黑曲霉中疏水蛋白基因种类分析[J]. 生命科学研究, 2017, 21(2): 101-105.
ZHANG Yan, XU Ke, ZHANG Jian-Guo. Analysis of Hydrophobin Genes from Aspergillus niger. Life Science Research, 2017, 21(2): 101-105.