Advances in the Identification of Drug Targets Based on Liquid Chromatography-Tandem Mass Spectrometry
National Health Commission Key Laboratory of Cancer Proteomics, Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China
Abstract：A drug target is a specific structure in a cell, tissue or organ that interacts with a drug to make it work. Comprehensive identification of drug targets is essential to understanding the mechanism of action of drugs and their potential side effects. Currently, widely used drug target identification and validation tech-niques, such as activity-based protein profiling (ABPP), compound-centric chemical proteomics (CCCP), re-quire the modification of small-molecule compounds, which may reduce or alter the activity of drug mole-cules. Therefore, advanced techniques that do not require chemical modification of drug molecules, such as drug affinity responsive target stability (DARTS), cellular thermal shift assay (CETSA), and thermal proteome profiling (TPP), are becoming important tools for drug target research. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful tool for identification of drug target proteins. This paper reviewed drug target research techniques based on LC-MS/MS and future development of drug target identification.
金 金, 梁旭俊, 毕 武, 张鹏飞. 基于液相色谱-串联质谱技术鉴定药物靶标的研究进展[J]. 生命科学研究, 2023, 27(3): 275-282.
JIN Jin, LIANG Xujun, BI Wu, ZHANG Pengfei. Advances in the Identification of Drug Targets Based on Liquid Chromatography-Tandem Mass Spectrometry. Life Science Research, 2023, 27(3): 275-282.
