Abstract:Abstract: In order to explore the evaluation method of aerobic exercise capacity in zebrafish and lay a foun-dation for the research of zebrafish in the fields of sports medicine, environmental monitoring, genetics, pharmacology and so on, a swimming respirometer was used. The maximum oxygen consumption and relative critical swimming speed of wildtype zebrafish of different ages were determined based on the detection scheme designed. Meanwhile, a microblood lactic acid tester was used to detect the lactic acid level in ze-brafish blood under progressively increasing swimming speed. The results showed that the maximum oxygen consumption and relative critical swimming speed of 3-, 8- and 14-month-old zebrafish decreased with age, and the blood lactate thresholds of 3-, 8- and 14-month-old zebrafish were (2.98±0.86) mmol/L, (2.47±0.29) mmol/L and (3.83±0.25) mmol/L, respectively, which appeared at 60%, 80% and 80% of the critical swimming speed, respectively. The measurement techniques of maximum oxygen consumption and blood lactate threshold of zebrafish established in this study can be used to accurately evaluate the aerobic exercise capacity in ze-brafish.
引用本文:
杨 栋, 陈章林, 肖姜伶, 周作琼, 张 平, 彭夕洋, 郑 澜, 汤长发. 斑马鱼有氧运动能力评估方法的探究[J]. 生命科学研究, 2021, 25(6): 520-525.
YANG Dong, CHEN Zhang-lin, XIAO Jiang-ling, ZHOU Zuo-qiong, ZHANG Ping, PENG Xi-yang, ZHENG Lan, TANG Chang-fa. Research on the Evaluation Method of Aerobic Exercise Capacity in Zebrafish. Life Science Research, 2021, 25(6): 520-525.