Abstract:Abstract: In order to explore the characteristics of in vitro regeneration in pepper, in vitro plant regenera-tion from cotyledons was induced and optimized in tissue culture and the process of their development was examined histocytologically by the paraffin section method. The results showed that the suitable hormone for-mula for the induction of in vitro regeneration contained 3.0~9.0 mg/L ZT, 5.0~9.0 mg/L 6-BA and 0.05 mg/L IAA, with MB as the basic medium. The rate of in vitro regeneration of cotyledons reached 100%. The re-generated structures were leafy with a fast growth rate, most being rosette-shaped and a few strip-shaped (in-cluding very few opposite strip-shaped ones). The calli obtained from cotyledons originated from the upper epidermal cells and their inside parenchyma ones. Meristematic cell masses formed by cell division developed into leaflike structures, instead of normally differentiating into buds. Several adjacent meristematic cell masses were together developed into a rosette-like leafy structure, and the single meristematic cell mass into a strip-shaped one. Leafy structures were formed due to the defect of bud development of meristems during regeneration. Only very few opposite strip-shaped leafy structures differentiated into buds but still in the state of growth stagnation. After the leafy structures were pruned out and only 1~2 pairs of opposite strip-shaped ones remained, the regenerated buds from them elongated 4~5 cm with 88%~90% elongation rates, when grown for about 15 days on the elongation medium with a hormone formula containing 5.0~7.0 mg/L ZT, 7.0 mg/L 6-BA and 0.05 mg/L IAA. The experiments may provide some theoretical support for establish-ment of in vitro regeneration and genetic transformation systems in pepper.
引用本文:
高 艺, 魏绍琦, 匡雪梅, 邱义兰. 辣椒子叶离体再生及组织细胞学分析[J]. 生命科学研究, 2021, 25(5): 463-470.
GAO Yi, WEI Shao-qi, KUANG Xue-mei, QIU Yi-lan. In vitro Regeneration of Cotyledon and Histocytological Analysis in Pepper (Capsicum annuum L.). Life Science Research, 2021, 25(5): 463-470.