Abstract:Abstract: Strigolactones (SLs) are important phytohormones and rhizospheric signaling molecules that play key roles in plant growth, rhizosphere fungal symbiosis, seed germination of parasitic plants, etc. Methods for SL quantification are needed to further explore their biosynthesis, transport and perception mechanisms, regula-tion roles of plant growth, crosstalk with other plant hormones, analog identification, etc. To date, however, sensitive and specific methods are limited. Chromatography and mass spectrometry, which have been used for quantitative analysis of plant hormones and their analogs, are tedious, time-consuming and high-cost, and require sophisticated equipments as well as trained operators. Therefore both are not suitable for insitu and real-time measurements. Due to unique advantages including simple operation, rapid response, low cost, and the potential for continuous, insitu, real-time, and visual monitoring of analytes, biosensorbased methods are an alternative for quantifying phytohormone contents and spatio-temporal distribution. So far, SL biosensors have been developed based on synthetic biology and the perception mechanism for endogenous SLs (e.g., degradation of the recruited repressor protein, degradation or conformational change of the SL receptor after SL binding to the receptor). Through this technique, SLs can be quantified by detecting changes in fluores-cence ratio or intensity. This may be the main trend for SL quantitation in the future. Owing to unique ad-vantages in small molecule detection, aptamer-based biosensors are likely to further promote SL quantification methods. Studies on biosensors would contribute to revealing the mechanisms of SLs and their interactions with other plant hormones, and promoting directed genetic improvement of plant architecture and rhizosphere symbiosis, as well as biological and chemical control of rhizosphere parasitic weeds.
引用本文:
方 园, 姚瑞枫. 生物传感检测独脚金内酯的研究与展望[J]. 生命科学研究, 2021, 25(5): 455-462.
FANG Yuan, YAO Rui-feng. Progress and Prospects on Biosensor-based Detection of Strigolactones . Life Science Research, 2021, 25(5): 455-462.