Abstract:Abstract: The pollen development, pollination and fertilization of one-season rice in the middle and lower reaches of the Yangtze River are extremely vulnerable to high midsummer temperatures, a main natural fac-tor leading to unstable rice yields. Selecting and breeding varieties whose pollens are able to develop at high temperatures is the key to yield stability of one-season rice. In this study, 190 rice materials were compared in their pollen fertility under high temperature conditions. The results showed that 36 materials, including MZQA8, MCQA2, etc., were high temperature-resistant, with the stained pollen rates and seed-setting rates higher than 80% and 70%, respectively. There were 135 high temperature-sensitive materials, some of whose pollens were proved abortive by staining and seed-setting rates were 15% lower than those under normal conditions. Nineteen materials were extremely sensitive to the high temperature, with completely abortive pol-lens and 0 seed-setting rates, among which 4 belonged to the typical abortion type and 15 the pollen-free sterile type. Comparison of the mRNA expression levels of the UbL40 gene, which expresses in response to high temperatures and is adverse to pollen development, revealed that the relative expression levels of UbL401, UbL402b and UbL404c in the anthers of high temperature-resistant rice materials were significantly lower than those of high temperature-sensitive ones. The screened-out materials in the study, such as MZQA8, MC-QA2, and so on, are rice varieties with pollens being able to develop under high temperature conditions, and can be used as resources for breeding high temperature-resistant one-season rice varieties suitable for culti-vation in the middle and lower reaches of the Yangtze River. The relative low expression levels of UbL401, UbL402b and UbL404c under high temperature conditions may be an important molecular mechanism underlying the high temperature tolerance of rice pollen development.
引用本文:
王思瑶, 田清源, 张林安, 李佳馨, 郭沙沙, 毛丹丹, 陈良碧. 不同水稻花粉育性耐高温的差异比较研究[J]. 生命科学研究, 2021, 25(5): 400-405.
WANG Si-yao, TIAN Qing-yuan, ZHANG Lin-an, LI Jia-xin, GUO Sha-sha, MAO Dan-dan, CHEN Liang-bi. Comparative Study on Fertility and High Temperature Tolerance of Different Rice Pollens. Life Science Research, 2021, 25(5): 400-405.